Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sakai, K.; Kimata, K.; Sato, T.; Gotoh, M.; Narimatsu, H.; Shinomiya, K.; Watanabe, H.
    Chondroitin sulfate N-acetylgalactosaminyltransferase-1 plays a critical role in chondroitin sulfate synthesis in cartilage (2007), J. Biol. Chem., 282, 4152-4161.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.226 medicine CSGalNAcT-1 plays a critical role in chondroitin sulfate biosynthesis in cartilage Homo sapiens
2.4.1.226 medicine CSGalNAcT-1 plays a critical role in chondroitin sulphate biosynthesis in cartilage Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.174 LTC cells from rat chondrosarcoma are transfected with chondroitin sulfate N-acetylgalactosaminyltransferase-1 expression plasmid (about 80fold increase of chondroitin sulfate N-acetylgalactosaminyltransferase-1 RNA), metabolic albeling with [35S]sulfate show up to a 2.2fold increase in chondroitin sulfate levels in these cells, but the mRNA level of aggrecan core protein does not change. Aggrecan obained from chondroitin sulfate N-acetylgalactosaminyltransferase-1 overexpressing cells contain a larger amount of chondroitin sulfate, but the chondroitin sulfate chain length is similar to mock-transfected cells. Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.174 additional information in cmd mice (known as natural aggrecan-null mice) the heterozygote and homozygote cartilage exhibit about 50% and about 9% aggrecan gene transcription respectively. In the cmd heterozygote cartilage, chondroitin sulfate N-acetylgalactosaminyltransferase-1 expression is dimnished to about 30% that of the wild type. In the cmd homozygote cartilage, chondroitin sulfate N-acetylgalactosaminyltransferase-1 expression furhter decreases to a low level. Mus musculus
2.4.1.226 additional information adenoviral gene delivery of the enzyme into intervertebral discs of 4-month -old ICR mice displays a substantial increase in the level of chondroitin sulfate biosynthesis Homo sapiens
2.4.1.226 additional information overexpression of the enzyme in chondrocytic cells further enhances chondroitin sulfate biosynthesis but not that of the aggrecan core protein, indicating that the enzyme activity is not saturated in the cells and that aggrecan synthesized in the overexpressing cells is heavier than the native molecule Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.174 Homo sapiens
-
 transfected cells are injected into the cuadal intervertebral discs of 4-month old ICR mice, analyses at day seven after the injection show, that in vivo gene delivery of chondroitin sulfate N-acetylgalactosaminyltransferase-1 increases chondroitin sulfate biosynthesis in cartilage.
-
2.4.1.174 Mus musculus
-
-
-
2.4.1.175 Mus musculus
-
 mouse
-
2.4.1.226 Homo sapiens
-
-
-
2.4.1.226 Mus musculus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.174 UDP-N-acetyl-alpha-D-galactosamine + [protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine = UDP + [protein]-3-O-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine the enzyme mainly regulates CS synthesis in cartilage Mus musculus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.174 bone
-
 Homo sapiens
-
2.4.1.174 additional information the enzyme is expressed (at very high levels) in the prehypertrophic zone of the growth plate at E16.5, colocalized with aggrecan core protein. In ATDC5 cells the expression of CSGlcAT and CSGalNAcT-1 correlate well with that of aggrecan core protein. Real time RT-PCR show a high level of chondroitin sulfate N-acetylgalactosaminyltransferase-1 expression in cartilage at E18.5. Mus musculus
-
2.4.1.175 additional information the enzyme is expressed (at very low levels) in the prehypertrophic zone of the growth plate at E16.5, colocalized with aggrecan core protein. Mus musculus
-
2.4.1.226 cartilage by in situ hybridization and real time RT-PCR of developing cartilage, CSGal-NAcT-1 exhibits the highest level of expression among other glycosyltransferases involved in chondroitin sulphate synthesis Homo sapiens
-
2.4.1.226 cartilage in heterozygote and homozygote aggrecan-null cartilage where aggrecan transcription is decreased, CSGal-NAcT-1 transcription diminishs accordingly Mus musculus
-

Synonyms

EC Number Synonyms Comment Organism
2.4.1.174 CSGalNAcT-1
-
 Mus musculus
2.4.1.174 CSGalNAcT-1 chondroitin sulfate N-acetylgalactosaminyltransferase-1 Homo sapiens
2.4.1.175 CSGalNAcT-2
-
 Mus musculus
2.4.1.226 chondroitin sulfate N-acetylgalactosaminyltransferase-1
-
 Mus musculus
2.4.1.226 chondroitin sulfate N-acetylgalactosaminyltransferase-1
-
 Homo sapiens
2.4.1.226 CSGalNAcT-1
-
 Mus musculus
2.4.1.226 CSGalNAcT-1
-
 Homo sapiens